High-throughput sequencing, also known as Next-generation sequencing technology, enables sequencing and general read lengths of hundreds of thousands to millions of DNA molecules in parallel. Shorter is the sign.
High-throughput sequencing is a milestone in the evolution of sequencing technology, which enables simultaneous sequencing of millions of DNA molecules. This makes it possible to perform a detailed analysis of the transcriptome and genome of a species, so it is also called deepepsequencing or next generationsequencing (NGS).
According to the history of development, influence, sequencing principles and technology, there are mainly the following: Massively Parallel Signature Sequencing (MPSS), Polymeris Cloning (Plonony Sequencing), 454 Pyrosequencing (454 pyrosequencing) , Illumina (Solexa) sequencing, ABI SOLiD sequencing, ion semiconductor sequencing (Ion semiconductor sequencing), DNA nanoball sequencing, and the like.
Speaking of high-throughput sequencing, I believe that many people are not as special as Xiaobian. Today, everyone will follow the Xiaobian to understand the principle of high-throughput sequencing technology and its application. High-throughput sequencing is also known as next-generation sequencing technology and deep sequencing. It can sequence hundreds of thousands or even millions of DNA molecules at a time, and generally read shorter marks. It makes it a completely new look for the transcription and gene analysis of a species.
Currently, the high-throughput sequencing technology mainly refers to the second-generation sequencing technology introduced by 454 Lifescience, ABI and Illumian, and the single-molecule sequencing technology launched by Helicos HeliscopeTM and Pacific Biosciences. Compared to 96 sequencing capillary sequencing of traditional sequencing, high-throughput sequencing can read 400,000 to 4 million sequences in one experiment. The read length varies from 25bp to 450bp depending on the platform. Different sequencing platforms can read the number of bases ranging from 1G to 14G in one experiment, so the huge sequencing capability is unmatched by traditional sequencers.
Sequencing technology advances the development of scientific research. With the rapid development of second-generation sequencing technology, the scientific community has begun to use second-generation sequencing technology to solve biological problems. For example, de novo sequencing of a species that has no reference sequence at the genomic level to obtain a reference sequence for the species, laying the foundation for subsequent research and molecular breeding; genome-wide resequencing of species with reference sequences at the genome-wide level Scan and detect mutation sites and discover the molecular basis of individual differences. Full transcriptome sequencing at the transcriptome level for alternative splicing, single nucleotide polymorphisms in coding sequences, or small RNA sequencing to isolate new RNA molecules of specific size for sequencing microRNA molecule. At the transcriptome level, combined with chromatin immunoprecipitation and methylated DNA co-precipitation techniques, DNA regions and genomic methylation sites that bind to specific transcription factors are detected.
What needs to be specifically pointed out here is the application of second-generation sequencing combined with microarray technology-targeted sequence sequencing technology (TargetedResequencing). This technique first uses microarray technology to synthesize a large number of oligonucleotide probes that can complement a specific region of the genome to enrich for a specific segment and then use a second-generation sequencing technique. These segments were sequenced. Agilent and Nimblegen are currently available for sequence capture, and the most widely used is human exome capture sequencing. Scientists now believe that exome sequencing is more advantageous than whole-genome resequencing, not only because of lower costs, but also because the data analysis of exome sequencing is less computationally intensive and more directly integrated with biological phenotypes. .
The emergence of high-throughput sequencing technology can be said to be a very meaningful event in the field of genetic research. High-throughput sequencing has made considerable progress compared to sequencing of previous generations, and it has also been price-priced. A considerable improvement. High-throughput sequencing technology enables the acquisition of genetic codes for more other species at a lower cost of sequencing. It is believed that in the future development, high-throughput sequencing technology will only be more and more perfect. Xiaobian’s introduction today is here, I hope I can bring you some help.
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