How to ensure the quality and quality of ELISA kits - Database & Sql Blog Articles

**ELISA Reagent Quality Assurance: A Key to Accurate Lab Results** When it comes to laboratory reagents, product quality is a top concern for researchers. The accuracy and reliability of experimental results depend heavily on the quality of the reagents used. While the manufacturer plays a crucial role in ensuring quality, the operator's skill and attention also significantly impact the outcome. So, how can we ensure the quality and performance of ELISA products? ELISA diagnostic reagents have evolved over time, moving from synthetic peptides to genetically engineered antigens. However, due to historical practices, many still evaluate ELISA kits based on background clarity. Some manufacturers use single-segment genetic antigens or synthetic peptides to achieve a clean background, but this often leads to insufficient sensitivity and poor stability. ![ELISA Kit](http://i.bosscdn.com/blog/20/17/10/21164224912887.jpg) The foundation of high-quality ELISA reagents lies in the raw materials and production process. At Shanghai Hengyuan, we take quality seriously. Our kits are designed not only to meet industry standards but to exceed them. Here’s what sets our products apart: 1. **Clean Production Environment & Strict Control Procedures**: We maintain a controlled and hygienic environment throughout the manufacturing process to ensure consistent quality across all batches. 2. **High-Quality Raw Materials**: We source antibodies from the most reputable kit manufacturers globally. Other excipients are imported bioactive materials, ensuring superior performance. Unlike many domestic suppliers, our detection antibodies and HRP enzymes are pre-diluted, allowing direct sample addition without the need for customer dilution. Similarly, our TMB substrate is a single-component solution, eliminating the mixing step before use. 3. **Advanced Coating Technology**: Our coating machines offer high precision, with plate-to-plate variation within 5%, ensuring reliable and reproducible results. 4. **User-Friendly Design**: Our reagents are optimized for ease of use. For example, our sample diluent helps reduce interference from serum components, leading to more accurate results. If you're short on time or prefer expert assistance, Shanghai Hengyuan Bio offers free testing services. Our trained technicians can perform the ELISA test for you, ensuring optimal performance and reducing the risk of errors. **Tips for Better ELISA Experiments:** - Plan your experiments carefully to avoid long incubation times and unnecessary waiting during washing steps. - Prepare the chromogen just before use and avoid using expired solutions. The light blue TMB developer should be clearly visible. - When adding the developer, make sure it doesn’t spill. Avoid contact between A and B solutions and metal instruments. - When adding the stop solution, be careful to avoid air bubbles. - After adding the enzyme reagent, gently blot the microplate surface with a paper towel to remove excess liquid. Shanghai Lima Experimental Equipment Co., Ltd. offers stable and cost-effective ELISA kits. We provide free testing services to help you achieve accurate and reliable results. Whether you're a seasoned researcher or new to ELISA, we’re here to support you every step of the way. Feel free to reach out for more information or assistance.

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