The quality of laboratory reagents is a critical concern for researchers, as it directly impacts the accuracy and reliability of experimental results. The quality of the product comes from the manufacturer, while the success of the experiment also depends on the operator's skill. So, how can we ensure the quality and performance of ELISA products?
ELISA diagnostic reagents have evolved from synthetic peptides to genetically engineered antigens. Historically, the quality of ELISA kits was often judged by the background signal. As a result, some manufacturers use single-segment genetic antigens or synthetic peptide coatings to achieve a clean background. However, this approach may lead to insufficient sensitivity and poor stability.
The foundation of product quality lies in raw materials and production processes. Once the quality is guaranteed, the experimental outcomes naturally improve. Shanghai Hengyuan offers technical support to help users optimize their experiments. Our RD brand ELISA kit, developed by Shanghai Hengyuan, has several key advantages:
1. **Clean Production Environment**: We maintain strict control over our production procedures to ensure consistent quality across all batches.
2. **High-Quality Raw Materials**: We use antibodies from the most reputable global kit manufacturers. Except for certain salts, our other excipients are imported bioactive materials. This ensures better performance and reliability.
3. **Direct Dilution**: Our detection antibodies and HRP enzymes are provided in ready-to-use concentrations, eliminating the need for customer dilution. The TMB substrate is a single-component solution, avoiding the mixing step before use.
4. **Precise Coating**: Our coating machines offer high precision, with plate-to-plate variation within 5%, ensuring reliable and reproducible results.
**Tips for Better Results**:
- Plan your experiments carefully to avoid long washing times.
- Prepare the color developer just before use and do not use it past its expiration date.
- The light blue TMB developer should be visible to the naked eye.
- Avoid spilling the developer when loading the plates. Do not let A and B components come into contact with metal tools.
- When adding the stop solution, make sure there are no air bubbles.
- After adding the enzyme reagent, gently blot the microplate surface with a paper towel to remove excess liquid.
Shanghai Liming Experimental Equipment Co., Ltd. offers ELISA kits with stable quality and competitive pricing. We also provide free testing services. Feel free to reach out for more information and assistance.
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